Journal article
Imaging of dairy emulsionsviaa novel approach of transmission electron cryogenic microscopy using beam exposure
Kilian Daffner, Eric Hanssen, Ian Norton, Tom Mills, Lydia Ong, Sally L Gras
Soft Matter | Royal Society of Chemistry | Published : 2020
DOI: 10.1039/d0sm00582g
Abstract
Emulsions containing small fat and protein particles are difficult to differentiate but low dose transmission electron cryogenic microscopy can visualize and distinguish between these droplets or particles. A controlled increase in electron dose caused the sample to bubble and visibly degrade at 150 e− Å−2 for protein particles, allowing particle differentiation. This technique may be useful for the development of nanoemulsions, as well as nanostructured and 3D printed foods.
Grants
Awarded by Australian Research Council's Industrial Transformation Research Program (ITRP)
Awarded by Engineering and Physical Sciences Research Council
Funding Acknowledgements
This research was supported by the Australian Research Council's Industrial Transformation Research Program (ITRP) funding scheme (project number IH120100005) and the Engineering and Physical Sciences Research Council [grant number EP/N024818/1]. The authors would like to thank The Bio21 Molecular Science & Biotechnology Institute at The University of Melbourne for access to equipment. The cryo EM analysis was carried out at the Bio 21 Advanced Microscopy Facility, at The University of Melbourne. We acknowledge Unternehmensgruppe Theo Mueller for gifting the protein powders. The authors would like to thank Eddie Pelan and Fotis Spyropoulos for useful discussions and proofreading.